A Case of Doxycycline-induced Melanin in the Gingiva Tissue: Case Report.

BACKGROUND: Gingival pigmentation is a discoloration of the gingiva due to a variety of lesions and conditions associated with several endogenous and exogenous etiologic features. OBJECTIVE: The purpose of this study is to describe a report of gingival pigmentation in a patient who used doxycycline. CASE REPORT: A 21-year-old Caucasian female was under dermatological treatment and antibiotic therapy with doxycycline 100 mg (one time a day) for 90 days. She presented brown pigmentation at the gingival margin on the facial surfaces of the upper and lower anterior incisors and premolars. The patient was evaluated by immunohistochemical (S-100, Melan-A, and HMB-45) and histopathologic analyses, and clinical history. Blood levels of malondialdehyde (MDA), glutathione (GSH), and superoxide dismutase (SOD) were analyzed by UV/Vis spectroscopy. The adrenaline, noradrenaline, and dopamine in blood were analyzed by high-performance liquid chromatography (HPLC); dehidroepiandrosterone (DHEA) in serum by radioimmunoassay; and luteinizing hormone (LH) and 25-Hydroxyvitamin D by chemiluminescence. Hematoxylin-eosin stained sections revealed keratinocytes with pigment compatible with melanin. The Fontana-Masson staining was positive in melanophages and in some basal keratinocytes. S-100, Melan A and HMB-45 were confirmed as positive markers of melanocytic differentiation in gingival tissue. We observed a significant increase in malondialdehyde (p˂0.05) and a decrease in superoxide dismutase levels (p˂0.05). The dopamine value was found to be 15 pg/ml (reference value ≤ 10 pg/ml). CONCLUSION: The use of doxycycline is associated with an increase in oxidative stress and of dopamine with melanin pigments in the gingival tissue. This case report showed a cause-effect relationship between exposure to doxycycline and pigmentation of the marginal gingiva.

Comparação de duas técnicas cirúrgicas para recobrimento radicular de recessões unitárias: ensaio clínico paralelo, randomizado e duplo cego / Comparison of two surgical techniques for root coverage of single-unit recessions: parallel, randomized, double-blind clinical trial

As crescentes demandas dos pacientes pelo tratamento das recessões gengivais trazem à tona questões terapêuticas clinicamente significativas, exigindo dos profissionais constante aperfeiçoamento em técnicas cirúrgicas cada vez menos invasivas e mais previsíveis. OBJETIVO: Comparar duas técnicas para recobrimento radicular em recessões gengivais unitárias, unilaterais, do tipo 1. METODOLOGIA: Este estudo clínico, paralelo, randomizado e duplo cego avaliou indivíduos com recessões gengivais unitárias, unilaterais, do tipo 1 (RT1), submetidos à cirurgia para recobrimento radicular, através da associação do enxerto de tecido conjuntivo subepitelial ao retalho posicionado coronalmente (grupo controle) e técnica de túnel (grupo teste). Os principais parâmetros avaliados foram profundidade de sondagem (PS), sangramento à sondagem (SS), nível clínico de inserção (NCI), recessão gengival (RG), faixa e espessura da mucosa ceratinizada (MC e EG), percentual de recobrimento radicular (RR) e fenótipo gengival (FG), além de fatores centrados no paciente (FCP), como dor pós-operatória, hipersensibilidade dentinária cervical (HSDC), estética, grau de satisfação e a qualidade de vida, intra e intergrupo, ao longo de 06 meses de acompanhamento. Os dados foram analisados estatisticamente através dos testes t emparelhado de Student, teste t para amostras independentes, Qui-quadrado, McNemar, Análise de Variância Split-Plot com pós-teste t de Student (α = 5%). RESULTADOS: 46 indivíduos finalizaram este estudo (controle: 23; teste: 23). O tempo de cirurgia foi maior para o grupo teste (controle: 40min ± 5,6; teste: 51min ± 5,9; p = 0,041). Foram observadas reduções estatisticamente significativas para a RG e ganho significativo do NCI, de MC e de EG na análise intragrupo, em ambos os grupos de tratamento, porém, sem diferenças entre as técnicas. O RR aumentou significativamente nos períodos avaliados, mas não foram observadas diferenças intergrupo (controle: 89,2%; teste: 86,5%; p = 0,069). A análise intragrupo revelou mudança de FG (controle: 95,65%; teste: 91,3%; p < 0,001). Ambos os protocolos de tratamento reduziram dor pós-operatória e HSDC, e proporcionaram melhora na estética, satisfação e na qualidade de vida (p < 0,001), sem diferenças entre as técnicas ao longo do tempo. CONCLUSÃO: Ambos os tratamentos apresentaram eficácia clínica semelhante em termos de recobrimento radicular e melhora dos FCP (AU).

The increasing demands of patients for the treatment of gingival recessions bring up clinically significant therapeutic issues, requiring professionals to constantly improve in less invasive and more predictable surgical techniques. AIM: To compare two root coverage techniques to treat single, unilateral, type 1 gingival recessions. METHOD: This parallel, randomized, doubleblind clinical trial evaluated individuals with single, unilateral, type 1 gingival recessions 1 (RT1), who underwent root coverage procedure with subepithelial connective tissue graft associated to a coronally advanced flap (control group) or a tunnel technique (test group). The main parameters evaluated were probing depth (PD), bleeding on probing (BoP), clinical attachment level (CAL), gingival recession (GR), heigth of keratinized tissue (KTH), gingival thickness (GT), percentage of root coverage (RC) and gingival phenotype (GP), in addition to patient-reported outcome measures (PROMs), such as postoperative pain, cervical dentin hypersensitivity (CDH), esthetics, degree of satisfaction and quality of life, intra and intergroup, throughout 06 months follow-up. Data were statistically analyzed using paired Student t-test, t-test for independent samples, Chi-square, McNemar, Split-Plot Analysis of Variance with post hoc t-test (α = 5%). RESULTS: 46 subjects completed this study (control: 23; test: 23). Surgery time was longer for the test group (control: 40min ± 5.6; test: 51min ± 5.9; p = 0.041). Statistically significant reductions for GR and significant gain for CAL, KTH and GT were observed in the intragroup analysis, in both treatment groups, however, without differences between techniques. The CR increased significantly, but no intergroup differences were observed (control: 89.2%; test: 86.5%; p = 0.069). Intragroup analysis revealed a change in GP (control: 95.65%; test: 91.3%; p < 0.001). Both treatment protocols reduced postoperative pain and CDH and improved esthetics, satisfaction and quality of life (p < 0.001), with no differences between the techniques over time. CONCLUSION: Both treatments showed similar clinical efficacy in terms of root coverage and improvement in PROMs (AU).

Impact of tooth loss due to periodontal disease on the prognosis of rehabilitation.

When periodontal disease is diagnosed, it is difficult to predict the clinical response of treatment of a tooth over time because the result of treatment is affected by several factors and will depend on the maintenance and support of periodontal treatment. Rehabilitation with removable dental prostheses, fixed prostheses, and dental implants makes it possible to restore the function and esthetics of patients with tooth loss due to periodontal disease. The predictive factors of tooth loss in periodontitis patients should be assessed by dentists to inform their clinical decision-making during dental treatment planning. This will provide detailed individualized information and level of risk of patients considered suitable for dental rehabilitation. Therefore, the aim of this article was to review the subject of "Impact of tooth loss due to periodontal disease on the prognosis of rehabilitation" and the effect of fixed, removable, and implant-supported prostheses in periodontal patients.

Impact of tooth loss due to periodontal disease on the prognosis of rehabilitation

Abstract When periodontal disease is diagnosed, it is difficult to predict the clinical response of treatment of a tooth over time because the result of treatment is affected by several factors and will depend on the maintenance and support of periodontal treatment. Rehabilitation with removable dental prostheses, fixed prostheses, and dental implants makes it possible to restore the function and esthetics of patients with tooth loss due to periodontal disease. The predictive factors of tooth loss in periodontitis patients should be assessed by dentists to inform their clinical decision-making during dental treatment planning. This will provide detailed individualized information and level of risk of patients considered suitable for dental rehabilitation. Therefore, the aim of this article was to review the subject of "Impact of tooth loss due to periodontal disease on the prognosis of rehabilitation" and the effect of fixed, removable, and implant-supported prostheses in periodontal patients.

COVID-19: Perspectives for the management of dental care and education.

The rapid and abrupt transmission pattern of the SARS-CoV-2 unleashed the current COVID-19 pandemic, as recognized by the World Health Organization in March 2020. Considering the high risk of transmission of the virus in dental environments and the specificities in clinical practice, COVID-19 posed immediate challenges for dental care and education. Due to the need to establish infection prevention and control guidance in dental health settings to enable a safe clinical practice, this review aims to list the challenges and perspectives in managing dental care in services and schools. This review employed materials collected from PubMed and the main guidelines and studies on the novel coronavirus to provide an overview of the clinical procedures and decisions made by health care personnel in dental offices and dental schools. We expect the COVID-19 scenario to promote significant changes in clinical practice and dental education; dentists should seek specific and particular regulations for dental practice established by their state or country. Biosafety checklists are strongly recommended for appointments at dental services and face-to-face activities in dental schools.

Assessment of gingival phenotype through periodontal and crown characteristics: a cluster analysis.

OBJECTIVE: To evaluate gingival phenotypes in 100 subjects (n = 100), with regard to maxillary central incisors and surrounding periodontium in an observational diagnostic study. MATERIALS AND METHODS: Individuals were grouped based on: Probing depth (PD); keratinized mucosa (KM); Ratio Height/Width Crown (CH/CW), papilla area (PA), gingival thickness (GT) and Gingival Volume (GV). ANOVA and chi-square tests were performed with a significance level of 5%. Results: PD (p=0.860) and CH/CW (p=0.086) were not statistically significant. Cluster analysis identified three groups: Cluster I (n = 32) had the lowest values: KM (4.54mm), GT (0.83mm), PA (15.64mm²) and GV (3.80mm³); Cluster II (n = 43) presented KM (8.02mm); GT (1.40mm); GV (11.18mm³) and PA (14.10mm²); Cluster III (n = 19) exhibited an average KM of 5.57mm, GT (1.14mm), PA (20.08mm²) and GV (6.48mm³). RESULTS: Cluster I was characterized as a thin phenotype; cluster II as thick, and cluster III, as an intermediate. Significant associations were found when transparency on probing was compared among clusters (p less than 0.05) and gingival exposure when smiling (p less than 0.05). CONCLUSION: Thin phenotype was found in 34.04% of the sample (cluster I), thick phenotype in 45.75% (cluster II) and intermediate phenotype in 20.21% (Cluster III)..

Distribuição das características clínicas do fenótipo gengival em pacientes saudáveis / Distribution of clinical characteristics of gingival phenotype in healthy patients

Resumo Introdução A determinação do fenótipo gengival possibilita compreender melhor as variações e diferenças clínicas intra e interindividuais dos tecidos periodontais saudáveis. Objetivo O objetivo deste estudo foi determinar os perfis do fenótipo gengival e compará-los em diferentes regiões da cavidade oral, levando em consideração parâmetros clínicos relacionados aos tecidos moles circunjacentes nos dentes anteriores superiores e inferiores. Material e método Noventa e um pacientes com saúde ​​periodontal foram examinados e os parâmetros clínicos profundidade de sondagem (PS), largura de mucosa ceratinizada (MC), espessura gengival em ambos incisivos centrais superiores (EG) e fenótipo gengival (FG) foram coletados nos dentes anteriores superiores e inferiores. Os dados foram analisados ​​estatisticamente pelos testes t-Student pareado e independente, Correlação de Pearson e Qui-quadrado, com um nível de significância de 5%. Resultado O fenótipo gengival espesso predominou nos dentes anteriores superiores (60,5%), enquanto o fenótipo fino foi mais prevalente nos dentes anteriores inferiores (84,6%). Diferenças estatisticamente significativas foram encontradas para os parâmetros largura de mucosa ceratinizada e espessura gengival em relação aos fenótipos gengivais superiores e inferiores (p<0,05). Não houve diferença estatística entre profundidade de sondagem e o fenótipo gengival nos dentes superiores e inferiores. Conclusão Concluiu-se que o fenótipo gengival espesso é mais frequente nos dentes anteriores superiores e que o fenótipo fino é mais comum nos dentes anteriores inferiores, sendo mais frequente em indivíduos do sexo masculino. Os parâmetros largura de mucosa ceratinizada e espessura gengival mostraram-se adequados à determinação do fenótipo gengival.

Abstract Introduction Gingival phenotype determination enables a better understanding of intra and interindividual clinical variations in healthy periodontal tissues. Objective The aim of this study was to determine gingival phenotype profiles and compare these in different regions of the oral cavity, considering clinical parameters related to the surrounding soft tissue in the upper and lower front teeth. Material and method Ninety-one periodontally-healthy patients were examined and clinical parameters such as probing depth (PD), keratinized tissue width (KW), gingival thickness at both maxillary central incisors (GT) and gingival phenotype (GP) were determined for the upper and lower front teeth. Data were statistically analyzed by paired and independent t-Student tests, Pearson Correlation and the Chi-square test, using a significance level of 5%. Result The thick gingival phenotype predominated in the upper anterior teeth (60.5%), while the thin phenotype was more prevalent in the lower anterior teeth (84.6%). Statistically significant differences were found for keratinized tissue and gingival thickness parameters, in relation to the upper and lower gingival phenotypes (p<0.05). There were no statistical significances for probing depth and gingival phenotype in the upper and lower teeth. Conclusion It was concluded that the thick phenotype is more frequent in the upper anterior teeth and that the thin phenotype is more common in the lower anterior teeth, and more frequent in males. The keratinized tissue and gingival thickness parameters proved to be suitable for the determination of gingival phenotype.

Determinação do biótipo periodontal através da análise de fotografias intra-orais / Determination of periodontal biotype through analysis of intra-oral photographs

Objetivo: Caracterizar os Biótipos Periodontais (BP) por meio de fotografias, além de verificar a concordância com o método clínico de determinação, bem como com diferentes profissionais em Odontologia. Material e método: A avaliação clínica intra-bucal de 34 pacientes (n=34) e verificação de fotografias padronizadas foram realizadas para caracterização dicotômica do BP em fino e espesso. A avaliação subjetiva, por meio da fotografia, foi comparada ao método clínico da transparência à sondagem. A determinação do BP por meio de fotografias foi realizada através da aplicação de um questionário contendo três categorias (fino, espesso e intermediário), direcionadas a 90 cirurgiões-dentistas divididos igualmente em três grupos: clínicos gerais, periodontistas e protesistas. A concordância em ambas as comparações foi avaliada por meio do Teste Kappa, além dos testes de sensibilidade e especificidade. Resultado: Na comparação entre o método clínico e o fotográfico, não houve concordância (K = 0,264, p = 0,113). Na comparação entre os grupos dos profissionais, foi encontrada uma baixa concordância (kappa = 0,160; p<0,05) entre os periodontistas e protesisas, bem como entre protesistas e clínicos gerais (kappa = 0,110; p = 0,142). Houve maior concordância, ainda que fraca, entre periodontistas e clínicos gerais (kappa = 0,291; p = 0,00). O questionário obteve um valor de sensibilidade e especificidade maior para o diagnóstico do BP espesso. Conclusão: A avaliação clínica do paciente por meio dos parâmetros clínicos ainda é necessária para caracterizar o BP, visto que a determinação usando-se análise visual por meio do fotografia, independentemente da especialidade, não foi efetiva, embora o BP espesso tenha sido o mais facilmente identificado.

Objective: To characterize the Periodontal Biotypes (PB) through photographs and verify the agreement with the clinical method of determination as well as with different Dental professionals. Material and method: Standardized photographs and intraoral clinical evaluation of 34 patients (n = 34) were performed for dichotomous characterization of PB in thin and thick. The subjective assessment, through photography, was compared to clinical method of probing transparency. The PB determination by photographs was performed through the application of a questionnaire containing three categories (thin, thick intermediate) directed to 90 dental professionals. They were equally divided into 3 groups: general practitioners, periodontists and prosthodontists. The concordance in both comparisons was evaluated through the Kappa Test, in addition to the sensitivity and specificity tests. Result: In the comparison between the clinical and the photographic method, there was no agreement (K = 0.264, p = 0.113). However, in the comparison between the groups of professionals, a low agreement (kappa = 0.160; p <0.05) was found between periodontists and prothesis, as well as between prosthesis and general practitioners (kappa = 0.110; p = 0.142). Greater agreement, although weak, between periodontists and general practitioners (kappa = 0.291, p = 0.00). The questionnaire obtained a higher sensitivity and specificity value for the diagnosis of thick PB. Conclusion: The evaluation of patients through clinical parameters is still necessary to characterize PB, since the determination using visual analysis using photography, regardless of specialty, was not effective, although thick PB was the most easily identified.